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Increased throughput in proteomic experiments can improve accessibility of proteomic platforms, reduce costs, and facilitate new approaches in systems biology and biomedical research. Here we propose combination of analytical flow rate chromatography with ion mobility separation of peptide ions, data-independent acquisition, and data analysis with the DIA-NN software suite, to achieve high-quality proteomic experiments from limited sample amounts, at a throughput of up to 400 samples per day. For instance, when benchmarking our workflow using a 500-µL/min flow rate and 3-min chromatographic gradients, we report the quantification of 5211 proteins from 2 µg of a mammalian cell-line standard at high quantitative accuracy and precision. We further used this platform to analyze blood plasma samples from a cohort of COVID-19 inpatients, using a 3-min chromatographic gradient and alternating column regeneration on a dual pump system. The method delivered a comprehensive view of the COVID-19 plasma proteome, allowing classification of the patients according to disease severity and revealing plasma biomarker candidates.
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In recent years, the interest in media representations of migrants and the media as a space for participation has increased within the field of migration studies. Yet, most scholars' attention is focused on immigrants and the media in destination countries, while less attention is paid to origin countries and emigrants' representation. Taking advantage of the increased attention paid to migrants and migration during the first phase of the COVID-19 pandemic, we investigated the media representations of Romanian migrants in agriculture who work in other European countries and interpreted how their voices could be heard through media accounts. Through content analysis, we investigated a sample of 297 articles published between 1st April and 31st May 2020 on the websites of the six most visible Romanian media outlets. This study contributes to the existing knowledge on media representations of Romanian migrants by documenting a series of tendencies, including an event-oriented approach, oversimplified representations of migration, massification and schematisation of migrant representations, and the high sensitivity to reports from destination countries' media on Romanian migrants. Our analysis reveals that the approach taken to reporting on migration during the COVID-19 pandemic, at least during its first phase, highly depended on the existing, institutionalised modes of media reporting on migration. Copyright © 2022 CSIC.
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The recent and sudden outbreak of monkeypox in numerous non-endemic countries requires expanding its surveillance immediately and understanding its origin and spread. As learned from the COVID-19 pandemic, appropriate detection techniques are crucial to achieving such a goal. Mass spectrometry has the advantages of a rapid response, low analytical interferences, better precision, and easier multiplexing to detect various pathogens and their variants. In this proteomic dataset, we report experimental data on the proteome of the monkeypox virus (MPXV) recorded by state-of-the-art shotgun proteomics, including data-dependent and data-independent acquisition for comprehensive coverage. We highlighted 152 viral proteins, corresponding to an overall proteome coverage of 79.5 %. Among the 1371 viral peptides detected, 35 peptides with the most intense signals in mass spectrometry were selected, representing a subset of 13 viral proteins. Their relevance as potential candidate markers for virus detection by targeted mass spectrometry is discussed. This report should assist the rapid development of mass spectrometry-based tests to detect a pathogen of increasing concern.
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Introduction: Italy was the first EU country to be affected by COVID-19 outbreak. The sudden change in everyday life was challenging for children and young people (CYP) who rely heavily on peer connections for emotional and social support. Concerns for consequences in CYP with type 1 diabetes (T1D) rose. Objective(s): To compare the diabetes-specific health-related quality of life (D-HRQOL) of CYP with T1D and their parents and the glycemic control before and after COVID-19-related lockdown. Method(s): The Pediatric Quality of Life InventoryTM 3.0 Diabetes Module (PedsQLTM 3.0 DM) was used to evaluate the D-HRQOL. Patients who filled the D-HRQOL before lockdown (December 2019-February 2020;T0) were recruited in the study and filled the same survey immediately after the lockdown was stopped (June 2020;T1) during a routine outpatient or telemedicine visit. Data on diabetic ketoacidosis (DKA), severe hypoglycemic events, insulin therapy [dose and delivery method (MDI/CSII)], glucose monitoring method (SBGM/isCGM/rtCGM), glycosylate hemoglobin (HbA1c), physical activity (h/week), and SARS-CoV-2 infection were also collected. Continuous and categorical data are reported as median and percentage, respectively. Result(s): Sixty-two CYP (males 48.4%;age: 12.6 [5.25-17.8] years;T1D duration 4.23 [0.45- 16.4] years;MDI 71%;rtCGM 52%, SBGM 29%, and isCGM 19%;telemedicine visit 56.5%) with T1D and their parents (60 mothers, 10 fathers) were enrolled. Patients' and fathers' scales scores did not significantly change from T0 to T1. Mothers' 'Diabetes symptoms' scale score increased (67.0 vs. 70.4;p=0.007). According to visit type (outpatient vs. telemedicine), glucose monitoring (SBGM vs. isCGM vs. rtCGM), and insulin therapy (MDI vs. CSII), D-HRQOL data were longitudinally comparable and no differences was found between groups. During lockdown no DKA, severe hypoglycemic events, and SARS-CoV-2 were recorded in patients. Despite the significant decrease of exercise from T0 to T1 (3.25 vs. 0.50 h/week;p<0.0001), glycemic control (HbA1c 58.5 vs. 57.9 mmol/mol) and total daily insulin dose (0.86 vs. 0.82 IU/kg/day) were unchanged. At T1, the lower the HbA1c, the better patients' 'Diabetes symptoms' (R=-0.41, p<0.001), 'Worry' (R=-0.27, p=0.032) scales, and total scores (R=-0.33, p=0.009). Similar results were found in parents. Conclusion(s): During COVID-19 pandemic-related lockdown the D-HRQOL did not change in CYP with T1D and their parents. During visits we stressed the importance to keep a balanced nutritional program, according to new time spent on exercise, making diabetes managements easier and allowing the maintenance of good glycemic control without acute complications.
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INTRODUCTION: Tracheobronchial aspergillosis (TBA) can be a difficult diagnosis despite being seen in up to a third of critically ill patients with COVID-19 with a high mortality rate. We report a case of TBA found on bronchoscopy shortly after COVID-19 infection diagnosis. DESCRIPTION: A 49-year-old woman with diabetes presented with encephalopathy, nausea, and malaise and a 1-day history of COVID-19 infection. She was initially stable, on 2 liters of oxygen via nasal cannula. She had a WBC of 27,000, procalcitonin: 10.9 ng/ml along with diabetic ketoacidosis (DKA), hemoglobin A1C: 15.5%, and acute kidney injury. She was treated for DKA and started on Remdesivir and Dexamethasone for COVID-19 infection. DKA resolved within 24 hours, however, she became hypoxic, bradycardic and had a PEA arrest on second day. After resuscitation, her beta-glucan was 364 pg/ml and Micafungin was started. She then developed severe worsening compliance on the ventilator. CT scan of the chest was non-revealing. Bronchoscopy for concerns of airway obstruction showed diffuse black-green dense exudate in trachea and bilateral airways with acute inflammation, suggestive of aspergillosis, later confirmed on cultures. She was switched to Amphotericin B. Patient further declined and was transitioned to comfort care after 5 days. DISCUSSION: TBA mostly afflicts patients with neutropenia, leukemia, HIV, transplantation and >3 weeks steroid treatment. It is also described in ventilated patients with viral infections like influenza. COVID-19 patients are also found to be susceptible to developing TBA with reports of 19-33% of patients having aspergillus in their BAL with mortality rate up to 65%. The diagnosis is often elusive prompting redefining guidelines for diagnosis of TBA. Except for short course of steroids and uncontrolled diabetes, our case did not have any other risk factors for TBA. Her accelerated TBA course can be attributed to her COVID-19 and/or its treatment. The CT imaging and ventilator findings could have been explained by COVID-19 infection. The high peak pressures on the ventilator prompted further investigation by bronchoscopy that uncovered her aspergillus infection. Considering the high mortality rate of TBA, our case shows high level of suspicion and thorough workup is needed for its diagnosis.
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Introduction: Candida spp.accounts for 70%-80% of invasive bloodstream fungal infections.It is most commonly spread in long-term care facilities, caring for people with severe medical conditions. Patients hospitalized for COVID-19 are at risk for healthcare-associated infections like candidemia. Candida auris is an emerging, multidrug-resistant, healthcare-associated fungal pathogen. Candida auris is currently one of the most common clinical fungal pathogens, causing nosocomial infections. Due to its higher drug-resistance rate, C. auris is more difficult to treat, requires longer hospitalization periods, and results in higher morbidity and mortality than other Candida species. Aim and Objectives: To analyze the risk factors associated with C. auris candidemia in COVID-19 and post-COVID-19 patients at tertiary care center. Material(s) and Method(s): We prospectively analyzed all positive blood samples which were received in the Microbiology department at SGPGI, Lucknow for a period of 1 year (March 2020-March 2021).Blood samples were inoculated and cultured in BACTECBottles (BD) andincubated for 5days at 37degreeC.The bottles whichflagged positive, aGram's stain wasperformed and were sub-cultured on SDA for isolation of yeast colonies. Isolated yeasts were identified by phenotypic method and confirmed by MALDI-T OF MS. Demographics details of the patients were collected and recorded. The significant associated risk factors with C. auris candidemia were analyzed. Result(s): A total of 13 000 blood samples were received during the 1-year study period from different departments of the hospital.1.25% (n = 163) of the blood culture samples were positive for candidemia. Out of 163 Candida culture-positive blood samples, 27.61% (n = 45) were C. auris. A total of 64% (n = 29) C. auris candidemia was seen in non-COVID-19 patients, 31.1% (n = 14) in COVID-19patients, and twopatients had ahistory ofpost-COVID-19 infection.Theassociated risk factors included the use of broad-spectrum antibiotics, intravenous catheterization, underlying respiratory illness, mechanical ventilation, use of steroids, and dialysis. A total of 46.6% (n = 21) mortality was seen with C. auris candidemia. Conclusion(s): Candida auris candidemia continues to be a threat in hospitalized patients. This study shows prevalence of C. auris candidemia in COVID-19 and post-COVID-19 patients with 47% mortality. Candida auris is continuously reported from different departments in our institute, especially from intensive care units with high morbidity and mortality.An alertness, awareness and infection control practices by the healthcare personnel will help in early diagnosis and appropriate antifungal therapy and control the spread of C. auris.
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IntroductionPatients with chronic kidney disease (CKD) and COVID-19 are at high risk of adverse outcomes due to the presence of comorbidities. However, it is still unclear whether dialysis therapy is associated with a worse prognosis in patients infected with SARS-CoV-2. The objectives were to assess mortality and risk factors associated with a worse prognosis of these patients (e.g., age, sex, comorbidities, Intensive Care Admission [ICU] admission, and need for invasive mechanical ventilation [IMV]).MethodsAn observational, descriptive, retrospective study was conducted in the private healthcare maintenance organization (Unimed-BH) of Belo Horizonte and 33 surrounding cities in Brazil. We used data collected from the organization's database. We included adult inpatients with CKD on previous dialysis therapy who tested positive for COVID-19, from February 2020 to June 2021.ResultsDuring the period, 16182 patients were admitted to Unimed-BH with a diagnosis of COVID-19. Of these, 333 (2%) had dialysis CKD. Male patients were 180 (54%), age ranged from 22.85 to 95.75 years and the mean was 60.91 years. Of the 333 patients, 109 (32.7%) were admitted to the ICU, and 56 (16.8%) required IMV. Among the 14 comorbidities analysed, the mean number of comorbidities was 6, with 93 (27.9%) dyslipidaemia, 74 (22%) diabetic, 270 (81%) hypertensive, 25 (7.5%) asthmatic, 42 (12.6%) with chronic pulmonary disease (CPD) and 122 (36.6%) with congestive heart failure (CHF). There were 66 (19.8%) deaths, 29 (43.9%) were male, the mean age was 60.8 years, and 23 patients (34.8%) were elderly (>60 years). Among the patients who died, 55 (83.3%) were in the ICU and 46 (69.7%) on IMV. The mean number of comorbidities was 9.27 being 16 (24.2%) dyslipidaemia, 44 (66.6%) diabetic, 60 (90.9%) hypertensive, 5 (7%) asthmatic, 10 (15%) with CPD and 32 (48.5%) with CHF.ConclusionsDialysis patients appear more susceptible to unfavourable outcomes than the general population. Our findings are similar to those reported in the world literature which is still scarce. It is important to conduct more studies on this population.
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Introduction: Mississippi ranks second for childhood obesity prevalence (22%) above the national average of 16% with significant racial and health disparities. Since March 2020, stay-at home orders, virtual learning, stress, and poor lifestyle may have provided a perfect setup leading to new diagnosis of diabetes and accounting for record high hospitalizations. Objective(s): To determine the impact of the COVID-19 pandemic on the rate and clinical characteristics of newly diagnosed pediatric diabetes at University of Mississippi Medical Center. Method(s): Chart review and data comparison completed for patients with new-onset-diabetes between January 2019-February 2020 (pre pandemic);and between March 2020-October 2020 (pandemic). Result(s): Females were predominant in both groups (53% vs. 52%). There was no significant difference in the racial distribution in the two groups. A 34% were Whites while 61% were Africans Americans in the prepandemic group. The pandemic group comprised of 30% Whites and 63% African Americans. There was no significant difference in BMI percentile in the two groups. African Americans were disproportionately diagnosed with diabetes (64%). A 80% of obese patients had type 2 diabetes with 38% presenting in DKA, 3 patients in HHS. Four patients were COVID-19 positive on admission. Conclusion(s): Our observations corroborate the steep rise in the number of new-onset type 1 and type 2 diabetes, particularly in African Americans, in a highly prevalent obese pediatric population. Additional studies are required to determine the detailed effects of SARS-CoV-2 infection and pandemic associated psychosocial/ physical effects on development of diabetes in the pediatric population.
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Background: AKI is common in critically ill patients with COVID-19. The aim of this study was to evaluate the 30-and 60-day survival of patients with COVID-19 and AKI, treated in the ICU. We analysed two groups of patients: patients in the first group were treated with CRRT only, and patients in the second group were treated with CRRT plus hemoadsorption with Cytosorb cartridge. Method(s): This is a retrospective study of patients admitted with COVID-19 between March, 2020 and April, 2022 in all COVID ICUs of our hospital. Inflammatory and biochemical biomarkers at admission, length of ICU stay, and mortality at day 30 and day 60 after ICU admission were analysed. Result(s): One hundred two patients (mean age 68.1+/-8.1 years, 74.5% male) had AKI requiring CRRT, and 44 (43.1%) out of these patients were treated concomitantly with CRRT and Cytosorb. Of the prior concomitant diseases, 39 (38.2%) patients had diabetes, 75 (73.5%) had hypertension, 22 (21.6%) had heart failure, and 26 (25.5%) had chronic kidney disease. Patients treated with CRRT and Cytosorb were younger (64.7 vs. 70.6 years;p<0.001), had lower serum creatinine levels (294 vs. 405 mumol/L;p<0.001), urea levels (29 vs. 48 mmol/L;p < 0.001), higher levels of IL-6 (1754 vs. 385 pg/mL;p<0.001) and lactate dehydrogenase (8.8 vs.7.1;p=0.038). We found no statistically significant difference between the two groups for serum lactate, ferritin, D-dimer, C-reactive protein, and procalcitonin. Onset of treatment was earlier in patients treated with CRRT and Cytosorb than in patients treated with CRRT alone (11.4 vs. 18.2 days;p=0.005). Mean length of stay in ICU was 27.4+/-20.2 days, with no differences between the two groups. Mortality 30 and 60 days after ICU admission was in all patients 58.8% and 76.5%. In patients treated with CRRT alone, mortality at 30 and 60 days was 65.5% and 84.5%, and in patients treated with CRRT and Cytosorb, 50% and 65.9%. The number of patients who died 60 days after ICU admission was statistically significantly higher in the group of patients treated with CRRT alone (x2, p=0.029). The most common causes of death were sepsis and multiple organ failure (55.1%), acute respiratory failure (24.4%), and cardiac arrest (19.2%). Conclusion(s): CRRT and CytoSorb cartridge treatment results in improved 60-day survival in COVID-19 ICU patients with AKI.
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Introduction: Neurogenic hypotension can happen in the context of immunoglobulin light chain AD. The most serious feature is autonomic nervous system impairment, mainly characterized by severe refractory orthostatic hypotension. Amyloid deposition may be found in many organs and the patient can presents with cardiac, gastrointestinal, renal, and neurological symptoms, but rarely hypotension only. Here we describing a patient who presented with sever orthostatic hypotension, found to have renal AD. Case Description: 67 year old male with past medical history of type 2 diabetes, chronic kidney disease, coronary artery disease, colon cancer, and history of COVID-19 infection who presents to nephrology clinic after being referred by primary care provider for elevated Creatinine to 2.5 mg/dl (baseline around 1.2 mg/dl). on clinic visit found to have severe orthostatic hypotension. blood pressure (BP) supine 121/80 mmHg, heart rate (HR) 87 beat per minute (BPM) ;sitting 106/70 mmHg, HR-92 BPM;standing: 92/59 mmHg, HR-100 BPM, no other abnormalities on physical examination. and was admitted to the hospital for evaluation. upon admission to the hospital, pateint received IV fluid, found to have 11 gram protein on 24 hour urine collection laboratory work up showed positive ANA, immunofixation was positive for lambda chain. Fat pad biopsy without signs of AD. Bone marrow biopsy results were inconclusive. Renal biopsy was performed, findings consistent with AL AD. pateint to start treatment with hematology Discussion: The amyloidoses are a group of disorders in which soluble proteins deposit extracellularly in tissues as insoluble fibrils, causing organ dysfunction that is usually progressive. Renal amyloid is a major source of morbidity in affected individuals. When the kidney is involved, renal insufficiency is common, accounting for 47% of cases in a study. Proteinuria has been reported with full nephrotic syndrome in 25-68%. it usually progress to end stage renal disease if left untreated. in our case we are describing an unusual presentation of renal AD, in which the symptoms were orthostatic hypotension. renal function was below baseline but was improving with volume replacement, pateint was felt to have prerenal acute kidney injury. the significant amount of proteinuria prompted further testing. We feel that nephrotic syndrome from amyloid should be considered when patient presented with orthostatic hypotension.
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Background: An 80 years old male patient presented with urinary retention. He had lower urinary tract symptoms for the last two years. The patient was catheterized and subsequently developed gross hematuria. The patient was COVID- 19 positive. Method(s): The ultrasonography showed bladder wall thickening with bladder mass around the neck. The computerized tomography scan showed heterogeneously enhancing thickening in the left lateral wall of the urinary bladder and was suspected to be neoplastic. There was a suspicious heterogeneously enhancing lesion in the base of the prostate. His serum prostate-specific antigen level was 5.79 ng/ml. His repeated urine cytology for malignant cells was non-diagnostic. Result(s): The patient underwent transurethral resection of bladder tumour (TURBT) and transurethral resection of the prostate (TURPT). The TURBT biopsy showed florid cystitis cystica et glandularis and the TURPT biopsy showed adenomatous hyperplasia. Conclusion(s): The florid cystitis cystica et glandularis mimic a malignancy, and it is, therefore, important to consider it as a differential diagnosis while evaluating catheterized patients with lower urinary tract symptoms.
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Type 2 diabetes mellitus (T2DM) has been recognized as a risk factor for severe coronavirus disease 2019 (COVID-19) infection, and COVID-19 in diabetic patients is associated with a poor prognosis. New evidence suggests that patients with T2DM may experience diabetic ketoacidosis (DKA) or hyperglycemic hyperosmolar state (HHS) if infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, there is limited literature on new-onset diabetes in patients infected by SARS-CoV-2 without a history of diabetes. We present a case series of three patients who developed new-onset diabetes while suffering from acute COVID-19 infection; they presented with DKA even though they had no prior history or risk factors for diabetes. They remain persistently insulin-dependent several months post-recovery.
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Background: Diabetes education is an essential element to achieve treatment objectives in children with type 1 diabetes mellitus (T1D) and it often involves group educations. Due to government group gathering restrictions during the Covid-19 pandemic, face-to-face group education were not possible. An alternative means of delivering group education is needed in this special time. With that, a series of virtual education workshops were designed. Aim: The study aimed to review the experience in conducting virtual education workshops for children and families with T1D during the Covid-19 pandemic. Method: The virtual workshops consisted of warm-up and ice-breaking games led by play therapists, followed by diabetes-related education workshops conducted by diabetes nurses, paediatric endocrinologists, and dietitians. Patients and families were invited to fill in a standard feedback questionnaire afterwards. Results: In the period of July 2020 to June 2021, a total of 10 virtual workshops were organised. A total of 33 children (13 boys and 20 girls) attended the workshops. The mean age of these patients was 9.5 +/- 3.8 years and the duration of T1D was 4.0 +/-3.3 years. Their mean HbA1c were 8.2 +/- 1.5%. A total of 22 responses on the feedback questionnaires were received. Among those who responded, 68% respondents had attended the virtual workshops for more than 2 times. The majority (86%) agreed that they had acquired new diabetes related knowledge from the workshops and 86% found the workshops interesting and enjoyable. Most (95%) reported that they could listen to instruction clearly and communicate causally in the virtual workshops. Only 32% strongly agreed that virtual workshops were the same as face-to-face workshops and 27% reported that they would prefer zoom to face-to-face workshop in the future. The commonest reasons for not joining virtual workshop in the future included unstable internet connection and feeling uneasy in virtual environment. Discussion: A safe, socially distanced method of delivering group education for children and families with T1D during the pandemic was possible and well-received. Virtual education workshops could be an alternative tool in delivering diabetes education. However, this could not totally replace face-to-face therapy.
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BACKGROUND: Acetaminophen (APAP) is a worldwide antipyretic as well as an analgesic medication. It has been extensively utilized during the outbreak of coronavirus 2019 (COVID-19). APAP misuse would lead to liver injury. Diacerein (DIA), an anthraquinone derivative, has antioxidant and inflammatory properties. Hence, this study attempted to evaluate the impact of DIA treatment on liver injury induced by APAP and its influence on nuclear factor-κB (NF-κB) /toll-like receptor 4 (TLR4)/high mobility group box-1(HMGB-1) signaling as well as the expression of peroxisome proliferator-activated receptor-gamma (PPAR-γ) expression. METHODS: Male albino rats received 25 as well as 50 mg/kg/day DIA orally for seven days. One hour after the last administration, rats received APAP (1gm/kg, orally). For histopathological analysis, liver tissues and blood were collected, immunohistochemical (IHC) assay, biochemical assay, as well as quantitative real-time polymerase chain reaction (qRT-PCR). RESULTS: DIA markedly reduced liver injury markers and ameliorated histopathological changes. Moreover, DIA dose-dependently alleviated oxidative stress status caused by APAP administration along with inflammatory markers, including the level of interleukin-1 beta (IL-1ß), myeloperoxidase (MPO), tumor necrosis factor-alpha (TNF-α), and interleukin 6 (IL-6). Furthermore, DIA downregulated protein levels as well as mRNA of HMGB-1, TLR4, NF-κB p65 expression, and enhanced PPAR-γ expression. Moreover, DIA ameliorated apoptotic (Bax) and caspase-3 expressions and increased the anti-apoptotic (Bcl2) expression. CONCLUSIONS: This study demonstrated that DIA exerts anti-apoptotic, anti-inflammatory, and antioxidant properties against liver injury induced by APAP that is attributed to inhibition of the HMGB1/TLR4/NF-κB pathway, besides upregulation of the expression of PPAR-γ.
Subject(s)
COVID-19 , Chemical and Drug Induced Liver Injury , HMGB1 Protein , Acetaminophen , Animals , Anthraquinones/metabolism , Anthraquinones/pharmacology , Anthraquinones/therapeutic use , Antioxidants/metabolism , Antioxidants/pharmacology , Chemical and Drug Induced Liver Injury/metabolism , HMGB1 Protein/metabolism , Humans , Liver/metabolism , Male , NF-kappa B/metabolism , PPAR gamma/metabolism , Rats , Toll-Like Receptor 4/geneticsABSTRACT
The spike protein of SARS-CoV-2, the virus responsible for the global pandemic of COVID-19, is an abundant, heavily glycosylated surface protein that plays a key role in receptor binding and host cell fusion, and is the focus of all current vaccine development efforts. Variants of concern are now circulating worldwide that exhibit mutations in the spike protein. Protein sequence and glycosylation variations of the spike may affect viral fitness, antigenicity, and immune evasion. Global surveillance of the virus currently involves genome sequencing, but tracking emerging variants should include quantitative measurement of changes in site-specific glycosylation as well. In this work, we used data-dependent acquisition (DDA) and data-independent acquisition (DIA) mass spectrometry to quantitatively characterize the five N-linked glycosylation sites of the glycoprotein standard alpha-1-acid glycoprotein (AGP), as well as the 22 sites of the SARS-CoV-2 spike protein. We found that DIA compared favorably to DDA in sensitivity, resulting in more assignments of low-abundance glycopeptides. However, the reproducibility across replicates of DIA-identified glycopeptides was lower than that of DDA, possibly due to the difficulty of reliably assigning low-abundance glycopeptides confidently. The differences in the data acquired between the two methods suggest that DIA outperforms DDA in terms of glycoprotein coverage but that overall performance is a balance of sensitivity, selectivity, and statistical confidence in glycoproteomics. We assert that these analytical and bioinformatics methods for assigning and quantifying glycoforms would benefit the process of tracking viral variants as well as for vaccine development.
Subject(s)
Glycomics/methods , Mass Spectrometry/methods , Proteomics/methods , SARS-CoV-2/metabolism , Spike Glycoprotein, Coronavirus/chemistry , COVID-19/virology , Glycosylation , Humans , Limit of Detection , Reproducibility of Results , Spike Glycoprotein, Coronavirus/metabolismABSTRACT
In a recent issue of Nature Communications, we highlighted in-depth urine proteomic research in which significant immunosuppression was revealed in early SARS-CoV-2- infected patients 1. The application of urine in mapping the landscape of molecular changes closely associated with human diseases has been widely accepted. Herein, we take a systematic review of the published article from the perspective of both methodology and clinical significance.
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This article aims to analyze the contributions of the storytelling to mental health in the context of the Covid-19 pandemic. It is an intervention-research that places this story device as light health technology. It is committed to reduce distances, create bridges between people through investment in bonding and welcoming. Storytelling articulates literature and psychology, and is part of the toolbox that is necessary for the activation of expressive psychic forces of affections in the current scenario. The research database can be designated as a bank of stories that undergo a curatorial process, which is a fundamental methodology for linking topics covered in the stories and posting them on the project's Instagram. The videotaped stories were analyzed from mutual-care practices and the context-affection-text semantic nuclei, with emphasis on the contents of memory and death. The reactions of Instagram followers express affectionate messages to the storytellers. © 2020.